A chromosome-level genome assembly of the Chinese tupelo Nyssa sinensis

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作者
Xuchen Yang
Minghui Kang
Yanting Yang
Haifeng Xiong
Mingcheng Wang
Zhiyang Zhang
Zefu Wang
Haolin Wu
Tao Ma
Jianquan Liu
Zhenxiang Xi
机构
[1] College of Life Sciences,Key Laboratory of Bio
[2] Sichuan University,Resource and Eco
[3] College of Life Sciences,Environment of Ministry of Education
[4] Lanzhou University,State Key Laboratory of Grassland Agro
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摘要
The deciduous Chinese tupelo (Nyssa sinensis Oliv.) is a popular ornamental tree for the spectacular autumn leaf color. Here, using single-molecule sequencing and chromosome conformation capture data, we report a high-quality, chromosome-level genome assembly of N. sinensis. PacBio long reads were de novo assembled into 647 polished contigs with a total length of 1,001.42 megabases (Mb) and an N50 size of 3.62 Mb, which is in line with genome sizes estimated using flow cytometry and the k-mer analysis. These contigs were further clustered and ordered into 22 pseudo-chromosomes based on Hi-C data, matching the chromosome counts in Nyssa obtained from previous cytological studies. In addition, a total of 664.91 Mb of repetitive elements were identified and a total of 37,884 protein-coding genes were predicted in the genome of N. sinensis. All data were deposited in publicly available repositories, and should be a valuable resource for genomics, evolution, and conservation biology.
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