Tight junction proteins: a novel class of integral membrane proteinsExpression in human epidermis and in HaCaT keratinocytes

被引:0
|
作者
B. Tebbe
J. Mankertz
C. Schwarz
S. Amasheh
M. Fromm
C. Assaf
U. Schultz-Ehrenburg
H. Sánchez Ruderisch
J.-D. Schulzke
C. Orfanos
机构
[1] Department of Dermatology,
[2] University Medical Center Benjamin Franklin,undefined
[3] Free University of Berlin,undefined
[4] Fabeckstr. 60–62,undefined
[5] 14195 Berlin,undefined
[6] Department of Gastroenterology,undefined
[7] Infectious Diseases and Rheumatology,undefined
[8] University Medical Center Benjamin Franklin,undefined
[9] Free University of Berlin,undefined
[10] Berlin,undefined
[11] Department of Clinical Physiology,undefined
[12] University Medical Center Benjamin Franklin,undefined
[13] Free University of Berlin,undefined
[14] Berlin,undefined
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Tight junction proteins Epidermal barrier Skin HaCaT keratinocytes;
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摘要
Tight junction proteins comprise a novel group of integral membrane proteins necessary for cell-to-cell contacts and responsible for the barrier function in epithelial and endothelial cells in various tissues. The tight junction membrane domain contains at least three distinct proteins, named occludin, claudin and junctional adhesion molecule. Claudins are products of a gene family consisting of more than 20 members. We investigated mRNA expression of occludin and 13 different claudins in neonatal foreskin, adult skin and cultivated HaCaT keratinocytes by the Northern blot technique, and performed immunohistochemical staining of adult skin for occludin, claudin 1 and claudin 2. Occludin, claudin 1 and claudin 3 mRNAs were expressed in human neonatal and adult keratinocytes as well as in HaCaT keratinocytes. All other tested claudins were negative. Immunohistochemical staining of adult skin was positive for occludin in the intercellular space of the granular layer, and for claudin 1 in the inter-cellular space of the spinosum layer and basal layer, but negative for claudin 2 in all skin layers. Claudin 1 was also positive in the outer root sheath of hair follicles. Our results indicate that occludin, claudin 1 and claudin 3 are involved in cell-to-cell contacts between keratinocytes in human epidermis, although their functional importance remains unknown.
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页码:14 / 18
页数:4
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