Phylogenetic analysis of different isolates of Sulfobacillus spp. isolated from uranium-rich environments and recovery of genes using integron-specific primers

被引：0

作者：

Muhammad A. Ghauri

Ahmad M. Khalid

Susan Grant

Shaun Heaphy

William D. Grant

机构：

[1] University of Leicester,Department of Microbiology and Immunology, Maurice Shock Medical Sciences Building

[2] National Institute for Biotechnology and Genetic Engineering,Department of Microbiology and Immunology

[3] University of Leicester,undefined

来源：

Extremophiles | 2003年 / 7卷

关键词：

Acidophilic moderate thermophiles; Gene cassettes; Integrons; 16S rDNA analysis; Uranium rich environments;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The isolation and phylogenetic characterization of acidophilic moderate thermophilic bacteria from different locations of uranium mines and a uranium processing mill in Pakistan is reported. The dominant culturable bacteria found were related to Sulfobacillus thermosulfidooxidans in all the samples analyzed. Different strains displayed different levels of identity (95–97%) to16S rDNA of known strains of this species, indicating group heterogeneity. Genomic DNA from five isolates was subjected to amplification using integron-specific primers HS286 and HS287. Recovery of different integron-linked genes from one of the isolates indicated the usefulness of this approach for gene mining in place of traditional gene recovery methodologies.

引用

页码：341 / 345

页数：4