Isolation, characterization, and EGFP expression in the buffalo (Bubalus bubalis) mammary gland epithelial cell line

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作者
Ramakant Kaushik
Karn Pratap Singh
Archana Kumari
Manoj Kumar Singh
Radhey Shyam Manik
Prabhat Palta
Suresh Kumar Singla
Manmohan Singh Chauhan
机构
[1] National Dairy Research Institute,Embryo Biotechnology Lab, Animal Biotechnology Centre
[2] Banasthali University,Department of Bioscience and Biotechnology
关键词
Collagen; Beta-lactoglobulin; Lactogenic hormones; EGFP; Lobules-like structure;
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摘要
This study was aimed to establish a buffalo mammary epithelial cells (BuMECs) line and maintain it for long-term by subculturing. BuMECs isolated from lactating buffalo mammary glands were cultured on a collagen matrix gel. BuMECs expressed significant amounts of the epithelial cell specific marker cytokeratin 18 as determined by immunohistochemistry. The BuMECs displayed monolayer, cobble-stone morphology, and formed lumen-, dome-, and duct-like structures. Furthermore, they were capable of synthesizing CSN2, BLG, ACACA, and BTN1A1, showed viability after thawing and expressed milk protein genes. The enhanced green fluorescent protein gene was transferred successfully into the BuMECs using lipofection method and the transfected cells could be maintained for long-term in culture by subculturing.
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页码:1 / 7
页数:6
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