Electrochemical DNA detection of hepatitis E virus genotype 3 using PbS quantum dot labelling

被引：0
|
作者：
Duy Ba Ngo
Thanyarat Chaibun
Lee Su Yin
Benchaporn Lertanantawong
Werasak Surareungchai
机构：
[1] King Mongkut’s University of Technology Thonburi (KMUTT),School of Bioresources and Technology
[2] Mahidol University,Department of Biomedical Engineering, Faculty of Engineering
[3] AIMST University,Department of Biotechnology, Faculty of Applied Sciences
[4] King Mongkut’s University of Technology Thonburi,Nanoscience and Nanotechnology Graduate Program, Faculty of Science
来源：
Analytical and Bioanalytical Chemistry | 2021年 / 413卷
关键词：
PbS quantum dots; Functionalization; Electrochemical DNA sensor; HEV3;
D O I：
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学科分类号：
摘要：
The aim of this study was to develop a highly specific electrochemical DNA sensor using functionalized lead sulphide (PbS) quantum dots for hepatitis E virus genotype 3 (HEV3) DNA target detection. Functionalized-PbS quantum dots (QDs) were used as an electrochemical label for the detection of HEV3-DNA target by the technique of square wave anodic stripping voltammetry (SWASV). The functionalized-PbS quantum dots were characterized by UV-vis, FTIR, XRD, TEM and zeta potential techniques. As-prepared, functionalized-PbS quantum dots have an average size of 4.15 ± 1.35 nm. The detection platform exhibited LOD and LOQ values of 1.23 fM and 2.11 fM, respectively. HEV3-DNA target spiked serum is also reported.
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页码：1027 / 1037
页数：10
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