A fungal extracellular effector inactivates plant polygalacturonase-inhibiting protein

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作者
Wei Wei
Liangsheng Xu
Hao Peng
Wenjun Zhu
Kiwamu Tanaka
Jiasen Cheng
Karen A. Sanguinet
George Vandemark
Weidong Chen
机构
[1] Washington State University,Department of Plant Pathology
[2] Northwestern A&F University,State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection
[3] Washington State University,Department of Crop & Soil Sciences
[4] Wuhan Polytechnic University,School of Life Science and Technology
[5] Washington State University,Molecular Plant Science Program
[6] Huazhong Agricultural University,State Key Laboratory of Agricultural Microbiology
[7] Grain Legume Genetics and Physiology Research Unit,USDA Agricultural Research Service
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摘要
Plant pathogens degrade cell wall through secreted polygalacturonases (PGs) during infection. Plants counteract the PGs by producing PG-inhibiting proteins (PGIPs) for protection, reversibly binding fungal PGs, and mitigating their hydrolytic activities. To date, how fungal pathogens specifically overcome PGIP inhibition is unknown. Here, we report an effector, Sclerotinia sclerotiorum PGIP-INactivating Effector 1 (SsPINE1), which directly interacts with and functionally inactivates PGIP. S. sclerotiorum is a necrotrophic fungus that causes stem rot diseases on more than 600 plant species with tissue maceration being the most prominent symptom. SsPINE1 enhances S. sclerotiorum necrotrophic virulence by specifically interacting with host PGIPs to negate their polygalacturonase-inhibiting function via enhanced dissociation of PGIPs from PGs. Targeted deletion of SsPINE1 reduces the fungal virulence. Ectopic expression of SsPINE1 in plant reduces its resistance against S. sclerotiorum. Functional and genomic analyses reveal a conserved virulence mechanism of cognate PINE1 proteins in broad host range necrotrophic fungal pathogens.
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