Interaction between proliferating cell nuclear antigen (PCNA) and a DnaJ induced by DNA damage

被引:0
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作者
Taichi Yamamoto
Yoko Mori
Toyotaka Ishibashi
Yukinobu Uchiyama
Tadamasa Ueda
Tsuyu Ando
Junji Hashimoto
Seisuke Kimura
Kengo Sakaguchi
机构
[1] Tokyo University of Science,Department of Applied Biological Science, Faculty of Science and Technology
[2] National Institute of Agrobiological Sciences,undefined
[3] Institute of the Society for Techno-innovation of Agrobiological Sciences,undefined
来源
Journal of Plant Research | 2005年 / 118卷
关键词
Chaperone; DNA damage; DnaJ; PCNA;
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学科分类号
摘要
Proliferating cell nuclear antigen (PCNA) is an essential protein for both DNA replication and DNA repair. In the present study using two-hybrid analysis with PCNA from rice, Oryza sativa L. cv. Nipponbare (OsPCNA), we found that OsPCNA interacted with rice DnaJ protein. We have identified DnaJ and designated it as OsDnaJ. OsDnaJ was able to bind to OsPCNA in vitro. Transcripts of OsDnaJ were found to be strongly expressed in the proliferating cells. mRNA of DnaJ was induced by UV and DNA-damaging agents such as H2O2. The expression patterns of OsPCNA were almost the same as OsDnaJ. The relationship between OsPCNA and OsDnaJ is discussed.
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页码:91 / 97
页数:6
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