Enhanced rhamnolipid production by Pseudomonas aeruginosa under phosphate limitation

被引:0
|
作者
K. G. Clarke
F. Ballot
S. J. Reid
机构
[1] University of Stellenbosch,Department of Process Engineering
[2] University of Cape Town,Department of Molecular and Cell Biology
关键词
Antimicrobial; Phosphate limitation; Rhamnolipid;
D O I
暂无
中图分类号
学科分类号
摘要
Rhamnolipid biosurfactants are effective antimicrobial agents and provide a promising alternative to synthetic medicine. Rhamnolipid accumulation by Pseudomonas aeruginosa ATCC 9027, and associated antimicrobial activity, was quantified during phosphate limited culture. The onset of rhamnolipid production occurred below 0.35 mg phosphate/l. Thereafter rhamnolipid accumulated during phosphate exhaustion where nitrogen remained above 0.9 g/l. A maximum 4.261 g rhamnolipid/l (measured as 1.333 g rhamnose/l) was attained at a productivity of 0.013 g rhamnose/l/h. Rhamnolipid accumulation under conditions of phosphate exhaustion and nitrogen excess suggests a non-specificity of the limiting nutrient, and that rhamnolipids will be synthesised provided carbon is in excess of the metabolic capacity. Antimicrobial activity was demonstrated against Mycobacterium aurum, a surrogate for M. tuberculosis, the causal agent of most forms of tuberculosis, by a 45 mm zone of M. aurum inhibition around a well of supernatant containing 3.954 g rhamnolipid/l.
引用
收藏
页码:2179 / 2184
页数:5
相关论文
共 50 条
  • [1] Enhanced rhamnolipid production by Pseudomonas aeruginosa under phosphate limitation
    Clarke, K. G.
    Ballot, F.
    Reid, S. J.
    [J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2010, 26 (12): : 2179 - 2184
  • [2] The Effect of Phosphate Limitation on Rhamnolipid Production and Related Quorum Sensing Signal Molecules in Pseudomonas aeruginosa
    Bukavaz, Sebnem
    [J]. FLORA INFEKSIYON HASTALIKLARI VE KLINIK MIKROBIYOLOJI DERGISI, 2019, 24 (02): : 93 - 100
  • [3] Osmotic stress and phosphate limitation alter production of cell-to-cell signal molecules and rhamnolipid biosurfactant by Pseudomonas aeruginosa
    Bazire, A
    Dheilly, A
    Diab, F
    Morin, D
    Jebbar, M
    Haras, D
    Dufour, A
    [J]. FEMS MICROBIOLOGY LETTERS, 2005, 253 (01) : 125 - 131
  • [4] Heterologous production of Pseudomonas aeruginosa rhamnolipid under anaerobic conditions for microbial enhanced oil recovery
    Zhao, F.
    Shi, R.
    Zhao, J.
    Li, G.
    Bai, X.
    Han, S.
    Zhang, Y.
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2015, 118 (02) : 379 - 389
  • [5] Heterologous production of Pseudomonas aeruginosa rhamnolipid under anaerobic conditions for microbial enhanced oil recovery
    20231713972244
    [J]. Zhang, Ying (yzhang@iae.ac.cn), 1600, (118):
  • [6] Enhanced rhamnolipid production by Pseudomonas aeruginosa overexpressing estA in a simple medium
    Dobler, Leticia
    de Carvalho, Bruna Rocha
    Alves, Wilber de Sousa
    Neves, Bianca Cruz
    Guimaraes Freire, Denise Maria
    Almeida, Rodrigo Volcan
    [J]. PLOS ONE, 2017, 12 (08):
  • [7] Imaging and Analysis of Pseudomonas aeruginosa Swarming and Rhamnolipid Production
    Morris, Joshua D.
    Hewitt, Jessica L.
    Wolfe, Lawrence G.
    Kamatkar, Nachiket G.
    Chapman, Sarah M.
    Diener, Justin M.
    Courtney, Andrew J.
    Leevy, W. Matthew
    Shrout, Joshua D.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (23) : 8310 - 8317
  • [8] Gene regulation of rhamnolipid production in Pseudomonas aeruginosa - A review
    Reis, Rodrigo S.
    Pereira, Alyson G.
    Neves, Bianca C.
    Freire, Denise M. G.
    [J]. BIORESOURCE TECHNOLOGY, 2011, 102 (11) : 6377 - 6384
  • [9] Genetic improvement of rhamnolipid production from Pseudomonas aeruginosa
    Al-Gelawi, Majed Hussain
    Al-Makadci, Oday Adnan
    [J]. ARAB GULF JOURNAL OF SCIENTIFIC RESEARCH, 2007, 25 (1-2): : 16 - 22
  • [10] Virulence Induction in Pseudomonas aeruginosa under Inorganic Phosphate Limitation: a Proteomics Perspective
    Matilla, Miguel A.
    Udaondo, Zulema
    Maass, Sandra
    Becher, Dorte
    Krell, Tino
    [J]. MICROBIOLOGY SPECTRUM, 2022, 10 (06):