Optimization of extraction process of bioactive compounds from Bene hull using subcritical water

Bene hull contains antioxidant components. Optimum conditions for bioactive compound extraction processes from Bene hull using subcritical water with response surface methodology (RSM) were obtained. Temperature (110–200°C), processing time (30–60 min), and the water to Bene hull ratio (10:1–50:1) were the investigated factors. The optimal conditions for maximizing the antioxidant activity were 196.8°C for 52.6 min and a ratio of 43.6:1 for water to Bene hull. Under these conditions, the amount of polyphenolic compounds, the reduction power (RP) (EC50), and the DPPH free radical scavenging activity (RSA) (EC50) were predicted to be 2,284 mg of gallic acid/100 g of Bene hull, 0.2002 mg/mL, and 0.6284 mg/mL, respectively. HPLC analysis was used to identify the main phenolic compounds. The subcritical water extraction technique could be used as a beneficial method to obtain bioactive compounds from Bene hull.