Prevalence of Enterocytozoon bieneusi in post-weaned dairy calves in the eastern United States

被引：0
|
作者：
Mónica Santín
James M. Trout
Ronald Fayer
机构：
[1] United States Department of Agriculture,Environmental Microbial Safety Laboratory, Animal and Natural Resources Institute Agricultural Research Service
来源：
Parasitology Research | 2004年 / 93卷
关键词：
Fecal Specimen; Gene Sequence Data; Dairy Calf; Cattle Feces; Small Subunit rRNA Gene;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Fecal specimens were obtained from 3- to 8-month-old post-weaned dairy calves on farms in Vermont, New York, Pennsylvania, Maryland, Virginia, North Carolina, and Florida. After removal of fecal debris by sieving and density gradient centrifugation, 59 of 452 calves (13%) from 11 farms in six states were found positive for Enterocytozoon bieneusi by PCR and DNA sequence analysis. Based on gene sequence data this genotype of E. bieneusi found in post-weaned calves was 100% identical to that found in pre-weaned calves in North America and differed by only two positions in 1,069 base pairs from specimens analyzed from humans. However, compared with previous reports, the prevalence of E. bieneusi was significantly higher in post-weaned than in pre-weaned calves from many of the same farms.
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页码：287 / 289
页数：2
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