Stable Isotope-Assisted Plant Metabolomics: Investigation of Phenylalanine-Related Metabolic Response in Wheat Upon Treatment With the Fusarium Virulence Factor Deoxynivalenol

被引：29

作者：

Doppler, Maria ^{[1
]}

Kluger, Bernhard ^{[1
]}

Bueschl, Christoph ^{[1
]}

Steiner, Barbara ^{[2
]}

Buerstmayr, Hermann ^{[2
]}

Lemmens, Marc ^{[2
]}

Krska, Rudolf ^{[1
,3
]}

Adam, Gerhard ^{[4
]}

Schuhmacher, Rainer ^{[1
]}

机构：

[1] Univ Nat Resources & Life Sci Vienna BOKU, Dept Agrobiotechnol IFA Tulln, Inst Bioanalyt & Agrometabol, Tulln, Austria

[2] Univ Nat Resources & Life Sci Vienna BOKU, Dept Agrobiotechnol IFA Tulln, Inst Biotechnol Plant Prod, Tulln, Austria

[3] Queens Univ Belfast, Inst Global Food Secur, Sch Biol Sci, Belfast, Antrim, North Ireland

[4] Univ Nat Resources & Life Sci Vienna BOKU, Dept Appl Genet & Cell Biol DAGZ, Tulln, Austria

来源：

FRONTIERS IN PLANT SCIENCE | 2019年 / 10卷

基金：

奥地利科学基金会;

关键词：

Triticum aestivum; Fusarium graminearum; LC-HRMS; Fhb1; resistance QTL; HEAD BLIGHT RESISTANCE; HYDROXYCINNAMIC ACID-AMIDE; QUANTITATIVE TRAIT LOCI; MYCOTOXIN DEOXYNIVALENOL; PHENOLIC-COMPOUNDS; MASKED MYCOTOXINS; GENE-EXPRESSION; FERULIC ACID; GRAMINEARUM; BIOSYNTHESIS;

D O I：

10.3389/fpls.2019.01137

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The major Fusarium mycotoxin deoxynivalenol (DON) is a virulence factor in wheat and has also been shown to induce defense responses in host plant tissue. In this study, global and tracer labeling with C-13 were combined to annotate the overall metabolome of wheat spikes and to evaluate the response of phenylalanine-related pathways upon treatment with DON. At anthesis, spikes of resistant and susceptible cultivars as well as two related near isogenic wheat lines (NILs) differing in the presence/absence of the major resistance QTL Fhb1 were treated with 1 mg DON or water (control), and samples were collected at 0, 12, 24, 48, and 96 h after treatment (hat). A total of 172 Phe-derived wheat constituents were detected with our untargeted approach employing C-13-labeled phenylalanine and subsequently annotated as flavonoids, lignans, coumarins, benzoic acid derivatives, hydroxycinnamic acid amides (HCAAs), as well as peptides. Ninety-six hours after the DON treatment, up to 30% of the metabolites biosynthesized from Phe showed significantly increased levels compared to the control samples. Major metabolic changes included the formation of precursors of compounds implicated in cell wall reinforcement and presumed antifungal compounds. In addition, also dipeptides, which presumably are products of proteolytic degradation of truncated proteins generated in the presence of the toxin, were significantly more abundant upon DON treatment. An in-depth comparison of the two NILs with correlation clustering of time course profiles revealed some 70 DON-responsive Phe derivatives. While several flavonoids had constitutively different abundance levels between the two NILs differing in resistance, other Phe-derived metabolites such as HCAAs and hydroxycinnamoyl quinates were affected differently in the two NILs after treatment with DON. Our results suggest a strong activation of the general phenylpropanoid pathway and that coumaroyl-CoA is mainly diverted towards HCAAs in the presence of Fhb1, whereas the metabolic route to monolignol(-conjugates), lignans, and lignin seems to be favored in the absence of the Fhb1 resistance quantitative trait loci.

引用

页数：18