Solid-Phase Fragment Condensation for Synthesis of Peptides from the Immunodominant Sequence of β1-Adrenoreceptor

被引：1

作者：

Sidorova, M. V. ^{[1
]}

Palkeeva, M. E. ^{[1
]}

Az'muko, A. A. ^{[1
]}

Ovchinnikov, M. V. ^{[1
]}

Molokoedov, A. S. ^{[1
]}

Sharf, T. V. ^{[1
]}

Efremov, E. E. ^{[1
]}

Golitsyn, S. P. ^{[1
]}

机构：

[1] Russian Hlth Minist, Russian Cardiol Res & Prod Complex, Moscow 121552, Russia

来源：

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2017年 / 43卷 / 04期

关键词：

the second extracellular loop of the beta(1)-adrenoreceptor; solid-phase peptide synthesis; convergent peptide synthesis; fragment condensation; racemization of a C-terminal amino acid residue; AUTOANTIBODIES; CARDIOMYOPATHY; ANTIGEN; LOOP;

D O I：

10.1134/S1068162017040112

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The P26 peptide corresponding to the 197-222 sequence of the second extracellular loop of the beta(1)-adrenoreceptor (beta(1)-AR) was synthesized by solid-phase fragment condensation on the Wang polymer. Pentapeptide fragments were prepared on the 2-chlorotrityl resin. The racemization degree of the C-terminal alanine residue of the pentapeptide was experimentally evaluated for the synthetic H-Glu-Ser-Asp-Glu-Ala-Arg-OH hexapeptide beta(1)-DR-(202-207) which was prepared by the 5 + 1 fragment condensation with the use of various condensing agents. A content of the diastereoisomeric peptide in the products of the fragment condensation was determined by HPLC on a reversed phase. The D-alanine-containing hexapeptide was specially synthesized and used for a comparison. The minimum racemization degree of the C-terminal alanine residue was observed if complex F was applied to the synthesis of the hexapeptide.

引用

页码：351 / 358

页数：8

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[1] Solid-phase fragment condensation for synthesis of peptides from the immunodominant sequence of β1-adrenoreceptor
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M. E. Palkeeva
A. A. Az’muko
M. V. Ovchinnikov
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T. V. Sharf
E. E. Efremov
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