Regulation of thyroid cell volumes and fluid transport: opposite effects of TSH and iodide on cultured cells

被引:9
|
作者
Cauvi, D
Penel, C
Nlend, MC
Venot, N
Allasia, C
Chabaud, O
机构
[1] Fac Med Marseille, INSERM, U38, F-13385 Marseille 05, France
[2] Fac Pharm Marseille, CNRS, Unite Propre Rech Enseignement Super Associee 603, F-13385 Marseille, France
[3] Univ Mediterranee, Fac Med, Ctr Microscopie & Anal Images, F-13385 Marseille, France
关键词
cell size; cAMP; thyrotropin control; iodide control;
D O I
10.1152/ajpendo.2000.279.3.E546
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Cell volume regulation by thyrotropin (TSH) and iodide, the main effectors involved in thyroid function, was studied in cultured thyroid cells. The mean cell volume, determined by performing 3-D reconstitution on confocal microscopy optical slices from living octadecylrhodamine-labeled cells cultured with both TSH and iodide (control cells), was 3.73 +/- 0.06 pl. The absence of iodide resulted in cell hypertrophy (136% of control value) and the absence of TSH in cell shrinkage (81%). These changes mainly affected the cell heights. The effect of TSH on cell volume was mediated by cAMP. The proportion of cytosolic volume (3-O-methyl- D-glucose space vs. total volume) decreased in the absence of iodide (85% of control value) and increased in the absence of TSH (139%), whereas protein content showed the opposite changes (121 and 58%, respectively). The net apical-to-basal fluid transport was also inversely controlled by the two effectors. Iodide thus antagonizes TSH effects on cell volumes and fluid transport, probably via adenylylcyclase downregulation mechanisms. The absence of either iodide or TSH may mimic the imbalance occurring in pathological thyroids.
引用
收藏
页码:E546 / E553
页数:8
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