Detection of cholera cells using surface plasmon resonance sensor

In this report, we describe the application of colloidal gold particles for bacterial cell. detection using a surface plasmon resonance (SPR) sensor. A monoclonal antibody against V. cholere was immobilized onto the gold film of a sensor chip activated with N-hydroxysuccinimide. Optimal conditions for the immunochemical reaction for cholera detection was investigated with streptavidin coated colloidal gold particles and biotinylated monoclonal antibody against cholera cells. The sensitivity of the cholera cell assay with SPR sensor could be enhanced 1000 times by binding of anti-cholera monoclonal antibody-biotin conjugate and streptavidin coated colloidal gold suspension. From a viewpoint of sensitivity, SPR sensor is about 10 times more sensitive than ELISA. This result illustrates the feasibility of using the SPR sensor in bacterial cell detection.