Antibiofilm activity of amylase from Bacillus spp. against foodborne pathogens

New strategies have been currently developed for removal and control of biofilm-associated microbial infections. Therefore, it is aimed to research the antibiofilm activity of amylase from Bacillus spp. on biofilm forming Citrobacter spp. 1.3 and 2.5 foodborne pathogens in this study. The bacteria belonging to genus Bacillus were isolated from Kilis soil sample. These strains produced amylase were named as 2B, 5B and 8B. Compared to others, the extracellular amylase sample purified from 2B strain showed the highest specific activity (20.83 +/- 0.77 U mg(-1)) at 90 mg mL(-1) enzyme concentration. Molecular weight of the amylase was calculated as 45 kDa by SDS-PAGE and native PAGE. The pink colonies of foodborne pathogens indicating antibiofilm activity of amylase were followed on congo red agar during 6-24h incubation. Our results revealed that 2B amylase sample at the 90 mg mL(-1) concentration effectively inhibited the synthesis of exopolysaccharides of biofilm forming pathogens.