Introduction: Dental research is progressing worldwide. Recently, there has been a remarkable growth in dentistry due to the development of newer technologies, advancement in dental materials, and sophisticated diagnostic materials. So, a study was done to assess the awareness of opportunities and difficulties in conducting research among dental interns and postgraduate dental students. Methods: A cross-sectional survey using a self-administered questionnaire was conducted in Riyadh Elm University, Riyadh city, Saudi Arabia among dental interns and postgraduate dental students. A closed ended, structured questionnaire comprising of 21 items was used. The questionnaires consisted of demographic details, opportunities and difficulties when conducting research. Data was analysed using the SPSS IBM software version 23. Results: The topmost reported opportunity and difficulty in conducting research were awareness that publishing research will enhance the chance for admission to higher studies and lack of research, respectively. Interns are more likely to be aware of the opportunities in conducting research and postgraduates are more like to be aware of the difficulties. Conclusion: Within the limitation of the study, opportunities and difficulties expressed were to be taken seriously which has impact on the research outcome of the institution. Addressing these will help the students and there of institution to do better in the research field in future.