Production of paramylon, a β-1,3-glucan, by heterotrophic cultivation of Euglena gracilis on potato liquor

被引:26
|
作者
Santek, Bozidar [1 ]
Felski, Michael [2 ]
Friehs, Karl [2 ]
Lotz, Martin [3 ]
Flaschel, Erwin [2 ]
机构
[1] Univ Zagreb, Dept Biochem Engn, Fac Food Technol & Biotechnol, HR-10000 Zagreb, Pierottijeva, Croatia
[2] Univ Bielefeld, Fac Technol, Bielefeld, Germany
[3] Emslandstarke GmbH, Emlichheim, Germany
来源
ENGINEERING IN LIFE SCIENCES | 2010年 / 10卷 / 02期
关键词
beta-1,3-glucan; Batch cultivation; Euglena gracilis; Heterotrophic growth Potato liquor;
D O I
10.1002/elsc.200900077
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Euglena gracilis is shown to be able to grow on potato liquor as the main medium component leading to an interesting biotechnological product represented by paramylon - a beta-1,3-glucan - and, at the same time, revaluating an otherwise annoying waste stream of the potato-starch industry. Paramylon mass fractions of about 75% are obtained for biomass concentrations of 15 g/L during simple batch cultivation under heterotrophic conditions. Supplementation of the growth medium with glucose and the vitamins B(1) and B(12) are shown to improve growth rate as well as paramylon content. E. gracilis grows best at about 27.5 degrees C without requiring pH control.
引用
收藏
页码:165 / 170
页数:6
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