Hydrolytic enzyme activity of Paenibacillus sp strain B2 and effects of the antagonistic bacterium on cell integrity of two soil-borne pathogenic fungi

被引：106

作者：

Budi, SW ^{[1
]}

van Tuinen, D ^{[1
]}

Arnould, C ^{[1
]}

Dumas-Gaudot, E ^{[1
]}

Gianinazzi-Pearson, V ^{[1
]}

Gianinazzi, S ^{[1
]}

机构：

[1] INRA, Ctr Microbiol Sol & Environm, UMR INRA Univ Bourgogne BBCE IPM, F-21034 Dijon, France

来源：

APPLIED SOIL ECOLOGY | 2000年 / 15卷 / 02期

关键词：

hydrolytic enzymes; Paenibacillus sp strain B2; cell integrity; wall metabolism; Phytophthora parasitica; Fusarium oxysporum;

D O I：

10.1016/S0929-1393(00)00095-0

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

Paenibacillus sp. strain B2, isolated from the mycorrhizosphere of Sorghum bicolor and having an antagonistic activity towards soil-borne fungal pathogens, possessed extracellular cellulolytic, proteolytic, chitinolytic and pectinolytic enzyme activities. The eventual role of these lytic enzymes in cellular interactions between Paenibacillus sp. strain B2 and Phytophthora parasitica and Fusarium oxysporum was investigated by electron microscopy and molecular cytology. Electron microscopic observations showed that the presence of Paenibacillus sp. strain B2 resulted in disorganisation of cell walls and/or cell contents of P. parasitica and F. oxysporum. However, when P. parasitica was treated with commercial purified cellulase, protease, chitinase and pectinase, only protease had an inhibitory effect on mycelial growth. It is proposed that the inhibitory effect of Paenibacillus sp. strain B2 on the growth of soil-borne fungal pathogens is probably derived from more than one mechanism. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：191 / 199

页数：9