Fluorescence polarization immunoassay of colchicine

被引：12
|
作者：
Zvereva, Elena A. [1 ]
Zherdev, Anatoly, V [1 ]
Formanovsky, Andey A. [2 ]
Abuknesha, Ramadan A. [3 ]
Eremin, Sergei A. [1 ,4 ]
Dzantiev, Boris B. [1 ]
机构：
[1] Russian Acad Sci, AN Bach Inst Biochem, Res Ctr Biotechnol, Moscow 119071, Russia
[2] Russian Acad Sci, MM Shemyakin & Yu A Ovchinnikov Inst Bioorgan Che, Moscow 117997, Russia
[3] Kings Coll London, London WC2R 2LS, England
[4] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2018年 / 159卷
基金：
俄罗斯科学基金会;
关键词：
Fluorescence polarization immunoassay; Colchicine; TANDEM MASS-SPECTROMETRY; ANCIENT DRUG; HUMAN PLASMA; RADIOIMMUNOASSAY; PHARMACEUTICALS; DIAGNOSTICS; SERUM; MILK; HPLC;
D O I：
10.1016/j.jpba.2018.07.008
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In this study, a fluorescence polarization immunoassay (FPIA) technique was developed to determine colchicine (COL), an alkaloid of noxious plants of the order Liliales that is used in a number of medications to treat gout. An optimal combination of the polyclonal antibody and the antigen labelled with fluorescein isothiocyanate (FITC) was selected. Conditions for the competitive interaction of the antigen in the tested samples and its fluorophore conjugate (COL-FITC) with anti-COL antibodies were optimised, and the analytical characteristics of the assay were determined. The developed FPIA was characterised by a detection limit of 1.8 ng/mL and a detectable analyte concentration range of 4.1-74.3 ng/mL. The duration of the analysis was 10 min. The applicability of the developed FPIA for quality control of ready-made drug formulations and for the estimation of COL content in various matrices (urine, milk), with recovery values ranging from 79 to 108%, was demonstrated. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码：326 / 330
页数：5
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