Identification of Colletotrichum spp. isolated from strawberry in Zhejiang Province and Shanghai City, China

被引：50
|
作者：
Xie, Liu [1 ]
Zhang, Jing-ze [1 ]
Wan, Yao [1 ]
Hu, Dong-wei [1 ]
机构：
[1] Zhejiang Univ, Inst Biotechnol, Minist Agr,Coll Agr & Biotechnol, Key Lab Mol Biol Crop Pathogens & Insects, Hangzhou 310029, Zhejiang, Peoples R China
来源：
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B | 2010年 / 11卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Fragariaxananassa; Taxonomic identification; Colletotrichum acutatum; C; gloeosporioides; fragariae; MOLECULAR CHARACTERIZATION; GENETIC DIVERSITY; ACUTATUM; ANTHRACNOSE; SEQUENCES; PATHOGEN; HOSTS; PCR;
D O I：
10.1631/jzus.B0900174
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Strawberry anthracnose, caused by Colletotrichum spp., is a major disease of cultivated strawberry. This study identifies 31 isolates of Colletotrichum spp. which cause strawberry anthracnose in Zhejiang Province and Shanghai City, China. Eleven isolates were identified as C. acutatum, 10 as C. gloeosporioides and 10 as C. fragariae based on morphological characteristics, phylogenetic and sequence analyses. Species-specific polymerase chain reaction (PCR) and enzyme digestion further confirmed the identification of the Colletotrichum spp., demonstrating that these three species are currently the causal agents of strawberry anthracnose in the studied regions. Based on analysis of rDNA internal transcribed spacers (ITS) sequences, sequences of all C. acutatum were identical, and little genetic variability was observed between C. fragariae and C. gloeosporioides. However, the conservative nature of the MvnI specific site from isolates of C. gloeosporioides was confirmed, and this site could be used to differentiate C. gloeosporioides from C. fragariae.
引用
收藏
页码：61 / 70
页数：10
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