Porous lithium-doped hydroxyapatite scaffold seeded with hypoxia-preconditioned bone-marrow mesenchymal stem cells for bonetissue regeneration

被引:40
|
作者
Li, Donghai [1 ]
Liu Huifang [2 ,3 ]
Jinhai Zhao [1 ]
Zhouyuan Yang [1 ]
Xiaowei Xie [4 ]
Wei, Zhun [1 ]
Li, Dongzhe [5 ]
Kang, Pengde [1 ]
机构
[1] Sichuan Univ, West China Hosp, Dept Orthopaed, 37 Wainan Guoxue Rd, Chengdu 610041, Sichuan, Peoples R China
[2] Univ Elect Sci & Technol China, Sichuan Acad Med Sci, Dept Rehabil Med, Chengdu, Sichuan, Peoples R China
[3] Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Chengdu, Sichuan, Peoples R China
[4] Chongqing Med Univ, Dept Orthopaed, Affiliated Hosp 2, Chongqing 400010, Peoples R China
[5] Deyang Peoples Hosiptal, Dept Orthopaed, Deyang 618000, Peoples R China
关键词
bone regeneration; hydroxyapatite; lithium; hypoxia; bone-marrow mesenchymal stem cells; SUBSTITUTED BIOACTIVE GLASSES; CATENIN SIGNALING PATHWAY; FEMORAL-HEAD; PHOSPHATE CEMENT; OSTEONECROSIS; OSTEOGENESIS; ANGIOGENESIS; REPAIR; VEGF; VASCULARIZATION;
D O I
10.1088/1748-605X/aac627
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Hydroxyapatite (HA) is a commonly used biomaterial in bone-tissue engineering, but pureHAis deficient in osteoinduction. In this study, we fabricated scaffolds of lithium-dopedHA(Li-HA) and assess the bone generation enhancement of Li-HA scaffolds seeded with hypoxia-preconditioned bone-marrow mesenchymal stem cells (BMMSCs). We found that 1.5% Li-HA obtained optimal cell proliferation activity in vitro. Inanin vivo study, Li-HA/BMSCs enhanced new bone formation, reducing the GSK-3 beta and increasing the beta-catenin, but the angiogenic effect was not modified significantly. However, when the seeded BMMSCs had been preconditioned in hypoxia condition, the new bone formation was increased, with lower GSK-3 beta and higher beta-catenin amounts detected. The HIF-1 alpha secretion was up-regulated, and the vascular endothelial growth factor and CD31 expression increased. In conclusion, the bone scaffold developed from Li-dopedHAseeded with hypoxiapreconditioned BMMSCs exerted positive effect on activating the Wnt and HIF-1 alpha signal pathway, and showed good osteogenesis and angiogenesis potential. The composited scaffold contributed to an encouraging result in bone regeneration.
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页数:14
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