A novel application of thermo-responsive polymer to affinity precipitation of polysaccharide

被引：16

作者：

Pan, LC ^{[1
]}

Chien, CC ^{[1
]}

机构：

[1] Ind Technol Res Inst, Ctr Biomed Engn, Hsinchu 310, Taiwan

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2003年 / 55卷 / 01期

关键词：

thermo-responsive polymer; lectin; polysaccharide; biomolecular separation;

D O I：

10.1016/S0165-022X(02)00180-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The lectin receptors concanavalin A (ConA) and wheat germ lectin (WGL) were successfully conjugated to a thermo-responsive hydrogel polymer (poly-N-isopropylacrylamide) (PNIPAAm) as an affinity ligand. The coupling efficiencies of the ConA and WGL to PNIPAAm were 5.1% and 44%, respectively. These lectin receptor-polymer conjugates were then tested for their efficiency in purification of various polysaccharides or polysaccharide-containing compounds such as beta-glucan. Results indicated that these conjugates separated various polysaccharides from a complex mixture. The use of a thermo-responsive polymer in low-temperature purification of potentially heat-labile glycoproteins is advantageous. Additionally, other affinity ligands could be coupled to the polymer for separation of the respective bioactive molecules. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：87 / 94

页数：8

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