Crystallization and preliminary crystallographic study of DNA polymerase from Pyrococcus furiosus

被引：3

作者：

Nishida, Hirokazu

Tanabe, Maiko

Ishino, Yoshizumi

Oyama, Takuji

Morikawa, Kosuke

机构：

[1] Osaka Univ, Inst Prot Res, Open Lab Adv Biosci & Biotechnol, Suita, Osaka 5650874, Japan

[2] JST, BIRD, Fukuoka 8128581, Japan

[3] Kyushu Univ, Fac Agr, Dept Genet Resources Technol, Fukuoka 8128581, Japan

[4] Hitachi Ltd, Cent Res Lab, Kokubunji, Tokyo 1858601, Japan

来源：

PROTEIN AND PEPTIDE LETTERS | 2007年 / 14卷 / 04期

关键词：

DNA polymerase; crystallization; archaea;

D O I：

10.2174/092986607780363853

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new member of archaeal DNA polymerase from Pyrococcus furiosus was crystallized. Diffraction data to 3.1 A of the selenomethionine-derivatized crystal were collected, and preliminary crystallographic study has been completed. The crystal belongs to the space group C2 with unit cell parameters of a = 93.2 angstrom, b = 124.9 angstrom, c = 87.7 angstrom, a = 90 degrees, 0 = 109.7 degrees, and gamma = 90 degrees. Assuming the presence of one molecule in the asymmetric unit, the solvent content of the crystal is estimated to be 54%, corresponding to a Matthews coefficient V-M of 2.7 angstrom 3 Da(-1).

引用

页码：403 / 405

页数：3

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[1] Purification, crystallization and preliminary X-ray crystallographic analysis of Pyrococcus furiosus DNA polymerase
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[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 : 986 - 988
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[J]. PROTEIN EXPRESSION AND PURIFICATION, 1997, 11 (02) : 179 - 184
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Inoue, T.
Kai, Y.
Yuasa, T.
Nishioka, M.
Fujiwara, S.
Takagi, M.
Imanaka, T.
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[8] Crystallization and characterization of the prolidase from Pyrococcus furiosus
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Ishino, Y
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