Sphingobacterium paludis sp nov., isolated from wetland soil

A novel Gram-stain-negative bacteria, designated S37(T), was isolated from soil of the Xixi wetland, Zhejiang province, China. Cells of strain S37(T) were aerobic, non-motile rods. Growth occurred at 10-37 degrees C (optimum, 25 degrees C), pH 5.0-9.7 (optimum, pH 7.5) and with 0-6% (w/v) NaCl (optimum, 0.5%). Based on 16S rRNA gene sequence analysis, strain S37(T) was found to be a member of the genus Sphingobacterium and shared highest similarity with Sphingobacterium composd 4M24(T) (95.78%). The major fatty acids were summed feature 3 (iso-C-15:0 2-OH and/or C-16:1 omega 7c, iso-C-15:0 and iso-C-17:0 3-OH, and the DNA G + C content was 43.8 mol%. The predominant respiratory quinone was MK-7. Based on its phenotypic and chemotaxonomic characteristics and phylogenetic data, strain S37(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium paludis sp. nov. (type strain S37(T)=CGMCC 1.12801(T)=NBRC 110386(T)) is proposed.