Evaluation of Diagnostic Tests for Clostridium difficile Infection

被引：126
|
作者：
Swindells, Jonathan [1 ]
Brenwald, Nigel [1 ]
Reading, Nathan [1 ]
Oppenheim, Beryl [1 ]
机构：
[1] City Hosp, Dept Med Microbiol, Birmingham, W Midlands, England
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 02期
关键词：
REAL-TIME PCR; ENZYME-IMMUNOASSAY; TOXIGENIC CULTURE; TOXIN; ALGORITHM; DIARRHEA; ASSAY; CYTOTOXIN; ANTIGEN;
D O I：
10.1128/JCM.01579-09
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We evaluated toxigenic Clostridium difficile detection by a lateral flow assay for antigen and toxin, an enzyme immunoassay, and two commercial PCR methods. Compared to the cell cytotoxicity neutralization assay and toxigenic culture, both toxin detection methods lacked sensitivity. PCR following combined antigen and toxin detection provided the most useful diagnostic information.
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页码：606 / 608
页数：3
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