fpr, a Deficient Xer Recombination Site from a Salmonella Plasmid, Fails To Confer Stability by Dimer Resolution: Comparative Studies with the pJHCMW1 mwr Site

被引:7
|
作者
Tran, Tung [1 ,2 ]
Sherratt, David J. [2 ]
Tolmasky, Marcelo E. [1 ,2 ]
机构
[1] Calif State Univ Fullerton, Coll Nat Sci & Math, Dept Biol Sci, Ctr Appl Biotechnol Studies, Fullerton, CA 92834 USA
[2] Univ Oxford, Dept Biochem, Div Mol Genet, Oxford OX1 3QU, England
基金
美国国家卫生研究院; 英国惠康基金;
关键词
KLEBSIELLA-PNEUMONIAE; NUCLEOTIDE-SEQUENCE; CER; RESISTANCE; INSTABILITY; MUTAGENESIS; AMPICILLIN; REQUIRES; SYSTEM; TN1331;
D O I
10.1128/JB.01082-09
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Salmonella plasmid pFPTB1 includes a Tn3-like transposon and a Xer recombination site, fpr, which mediates site-specific recombination at efficiencies lower than those required for stabilizing a plasmid by dimer resolution. Mutagenesis and comparative studies with mwr, a site closely related to fpr, indicate that there is an interdependence of the sequences in the XerC binding region and the central region in Xer site-specific recombination sites.
引用
收藏
页码:883 / 887
页数:5
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  • [1] Osmoregulation of dimer resolution at the plasmid pJHCMW1 mwr locus by Escherichia coli XerCD recombination
    Pham, H
    Dery, KJ
    Sherratt, DJ
    Tolmasky, ME
    [J]. JOURNAL OF BACTERIOLOGY, 2002, 184 (06) : 1607 - 1616