PCR-based molecular markers for the fragrance gene in rice (Oryza sativa. L.)

被引：39
|
作者：
Garland, S [1 ]
Lewin, L
Blakeney, A
Reinke, R
Henry, R
机构：
[1] So Cross Univ, Ctr Plant Conservat Genet, Lismore, NSW 2480, Australia
[2] NSW Agr, Yanco Agr Inst, Yanco, NSW 2703, Australia
[3] BRI Australian Ltd, Grains Proc, N Ryde, NSW 2113, Australia
来源：
THEORETICAL AND APPLIED GENETICS | 2000年 / 101卷 / 03期
关键词：
fragrance; rice; PCR marker; Oryza sativa;
D O I：
10.1007/s001220051492
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The genomic DNA clone RG28, linked to the major fragrance gene of rice (fgr), was assessed for polymorphism in order to produce a PCR-based marker for fragrance. A small mono-nucleotide repeat, that was polymorphic between a pair of fragrant and non-fragrant cultivars, was identified and developed into a co-dominant PCR-based marker. The polymorphism-information-content determinations for three microsatellite markers, that have been genetically mapped near RG28, are also presented. These PCR-based markers will be highly useful in distinguishing fragrance-producing alleles from non-fragrance-producing alleles at the fgr locus.
引用
收藏
页码：364 / 371
页数：8
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