A novel and rapid approach to yeast differentiation using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

被引：7
|
作者：
Sherburn, RE [1 ]
Jenkins, RO [1 ]
机构：
[1] De Montfort Univ, Sch Mol Sci, Leicester LE1 9BH, Leics, England
来源：
SPECTROSCOPY-AN INTERNATIONAL JOURNAL | 2003年 / 17卷 / 01期
关键词：
D O I：
10.1155/2003/849530
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI-TOF-MS) was investigated as a method for the rapid identification of yeast cells. Following pretreatment of yeast samples with a cell wall digesting enzyme (lyticase), distinct and reproducible mass spectra over the m/z range 2,000 to 16,000 were obtained by MALDI-TOF-MS. Using an optimised procedure, characteristic mass spectra that distinguished between Candida spp. and between strains of Saccharomyces cerevisiae were produced. The approach offers the potential for rapid differentiation of yeasts in clinical diagnosis and in the fermentation industries.
引用
收藏
页码：31 / 38
页数：8
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