Human amniotic fluid derived mesenchymal stem cells cause an anti-cancer effect on breast cancer cell line in vitro

被引：22
|
作者：
Ghafarzadeh, M. [1 ]
Eatemadi, A. [2 ]
Fakhravar, Z. [3 ]
机构：
[1] Lorestan Univ Med Sci, Assalian Hosp, Ctr Obstet & Gynecol, Khorramabad, Iran
[2] Univ Tehran Med Sci, Sch Adv Sci Med, Dept Med Biotechnol, Tehran, Iran
[3] Lorestan Univ Med Sci, Razi Drug Appl Res Ctr, Khorramabad, Iran
来源：
CELLULAR AND MOLECULAR BIOLOGY | 2016年 / 62卷 / 06期
关键词：
Stem cell; T47D; co-culture; anticancer therapy; NANOG;
D O I：
10.14715/cmb/2016.62.6.19
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Human amniotic fluid stem cells (hAFSCs) have the ability to self-renew, and multipotent differentiation into three germ layer cells. We obtained 5 ml amniotic fluid from ten 16-20 week pregnant women undergoing amniocentesis. hAFSCs were isolated from all samples, co-cultured with T47D breast cancer cell line and characterized using flow cytometry and RT-PCR. After 3, 4 and 5 days, T47D and HSFCs viability were evaluated with MTT assay. After 5 days of co-culture T47D cells viability were decreased. Our findings showed that hAFSCs can release soluble factors in cell culture, causing an efficient anticancer effect.
引用
收藏
页码：102 / 106
页数：5
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