Reconstruction of the phylogeny of grasshoppers from the family acrididae (Orthoptera) based on the mitochondrial 16S ribosomal RNA gene sequence

The sequences of two mitochondrial 16S RNA gene fragments (137- and 174-bp in size) were determined in nine grasshopper species belonging to three Acrididae subfamilies. Phylogenetic reconstruction was performed using the sequences of twelve grasshopper species and the cricket Acheta domesticus sequence as an outgroup (some data were purchased from the Genbank Data Library (NCBI)). In the phylogenetic tree, the Acridinae and Locustinae formed compact groups. An unexpected position of Celes scalozubovi (Locustinae) within the subfamily Acridinae indicated its vague phylogeny. The Catantopinae species lied close to the base of the Acridinae. Almost all branches of phylogenetic trees were strongly (55 - 100%) supported by bootstrap analysis.