Development of a new culture medium for the rapid detection of Salmonella by indirect conductance measurements

The main difficulties in conductance medium development are to allow Salmonella to grow and produce a conductance signal while impeding-growth of related species such as Escherichia coli and Citrobacter freundii. Various selective agents were screened for these capacities and a new medium was derived, named KIMAN (Whitley Impedance Broth basal medium supplemented with three selective components: novobiocin, malachite green and potassium iodide). This medium supported the growth of Salmonella serotypes and inhibited non-salmonella strains in pure cultures.