Isothermal Detection of DNA by Beacon-Assisted Detection Amplification

被引：136
|
作者：
Connolly, Ashley R. [1 ]
Trau, Matt [1 ]
机构：
[1] Univ Queensland, Ctr Biomarker Res & Dev, AIBN, Brisbane, Qld 4072, Australia
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 15期
关键词：
biosensors; DNA detection; DNA nanomachines; molecular devices; nanobiotechnology; STRAND DISPLACEMENT AMPLIFICATION; NUCLEIC-ACIDS; POLYMERASE; REPLICATION; INVITRO;
D O I：
10.1002/anie.200906992
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Not bad at all: A simple biological circuit composed of two integrated molecular switches was developed for the detection and amplification of a specific nucleic acid in a biological extract. The nucleic acid is recognized by a molecular beacon, then a DNA polymerase and nicking endonuclease are used to rapidly amplify the signal for sensitive detection of the nucleic acid. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：2720 / 2723
页数：4
相关论文
共 50 条
  • [1] Rapid DNA detection by beacon-assisted detection amplification
    Ashley R Connolly
    Matt Trau
    Nature Protocols, 2011, 6 : 772 - 778
  • [2] Rapid DNA detection by beacon-assisted detection amplification
    Connolly, Ashley R.
    Trau, Matt
    NATURE PROTOCOLS, 2011, 6 (06) : 772 - 778
  • [3] Ultrasensitive detection of telomerase activity in lung cancer cells with quencher-free molecular beacon-assisted quadratic signal amplification
    Ma, Fei
    Wang, Ting-ting
    Jiang, Longhe
    Zhang, Chun-yang
    ANALYTICA CHIMICA ACTA, 2019, 1053 : 122 - 130
  • [4] Isothermal amplification detection of nucleic acids by a double-nicked beacon
    Shi, Chao
    Zhou, Meiling
    Pan, Mei
    Zhong, Guilin
    Ma, Cuiping
    ANALYTICAL BIOCHEMISTRY, 2016, 496 : 9 - 13
  • [5] Rapid isothermal amplification and visual detection of DNA
    Tan, E
    Wong, J
    Niemz, A
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2005, 230 : U409 - U409
  • [6] Enzymatic amplification detection of DNA based on "molecular beacon" biosensors
    Mao, Xun
    Jiang, Jianhui
    Xu, Xiangmin
    Chu, Xia
    Luo, Yan
    Shen, Guoli
    Yu, Ruqin
    BIOSENSORS & BIOELECTRONICS, 2008, 23 (10): : 1555 - 1561
  • [7] Detection of rare DNA targets by isothermal ramification amplification
    Zhang, DY
    Zhang, WD
    Li, XP
    Konomi, Y
    GENE, 2001, 274 (1-2) : 209 - 216
  • [8] Isothermal DNA amplification strategies for duplex microorganism detection
    Santiago-Felipe, Sara
    Antonio Tortajada-Genaro, Luis
    Morais, Sergi
    Puchades, Rosa
    Maquieira, Angel
    FOOD CHEMISTRY, 2015, 174 : 509 - 515
  • [9] Direct detection of microRNAs using isothermal amplification and molecular beacon with excellent sensitivity and specificity
    Zhang, Wancun
    Zhang, Qi
    Qian, Zhiyu
    Gu, Yueqing
    REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS IX, 2017, 10079
  • [10] Locked nucleic acid molecular beacon for multiplex detection of loop mediated isothermal amplification
    Bakthavathsalam, Padmavathy
    Longatte, Guillaume
    Jensen, Slade O.
    Manefield, Mike
    Gooding, J. Justin
    SENSORS AND ACTUATORS B-CHEMICAL, 2018, 268 : 255 - 263
12345