Characteristics of Collagen from Rohu (Labeo rohita) Skin

被引:18
|
作者
Savedboworn, Wanticha [1 ]
Kittiphattanabawon, Phanat [2 ]
Benjakul, Soottawat [3 ]
Sinthusamran, Sittichoke [3 ]
Kishimura, Hideki [4 ]
机构
[1] King Mongkuts Univ Technol North Bangkok, Fac Agroind, Dept Agroind Technol & Management, Muang, Thailand
[2] Thaksin Univ, Fac Technol & Community Dev, Dept Food Sci & Technol, Phatthalung 93210, Thailand
[3] Prince Songkla Univ, Fac Agroind, Dept Food Technol, Hat Yai, Thailand
[4] Hokkaido Univ, Res Fac Fisheries Sci, Lab Marine Prod & Food Sci, Sapporo, Hokkaido, Japan
关键词
Collagen; ASC; PSC; rohu; Labeo rohita; type I collagen; SOLUBLE COLLAGENS; PEPSIN; ACID; SPECTROSCOPY; EXTRACTION; GELATIN; SCALE; BONE;
D O I
10.1080/10498850.2015.1133747
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) were isolated from rohu skin with the yield of 64.2 and 6.8% (dry weight basis), respectively. Both collagens had glycine as the major amino acid with imino acid content of 196-202 residues/1,000 residues and were characterized as type I collagen with molecular composition of (1)(2)2-heterotrimer. Fourier transform infrared spectra of both collagens were similar, with no shift in wavenumber of all amide bands. The T-max value of ASC and PSC was 36.40 and 35.48 degrees C, respectively. The zero surface net charge of ASC and PSC was found at pH 5.9 and 5.3, respectively.
引用
收藏
页码:248 / 257
页数:10
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