Enantiomeric separation of Trimebutine maleate and ondansetron on amylose chiral stationary phase

A high performance liquid chromatographic method has been developed for the enantiomeric separation of trimebutine maleate (TBM) and ondansetron (OND) on a Chiralpak AD-H Chiral column under normal phase mode. The influences of the concentration of organic solvent, the proportion of glacial acetic acid and triethyamine, column temperature and flow rate on the enantiomeric separation were investigated. The optimal chromatographic conditions on AD-H column were hexane-isopropanol-glacial acetic acid-tniethyamine (97:3:0.1:0.3) as mobile phase with a flow rate of 0.6 mL/min of TBM and hexane-isopropanol-glacial acetic acid-triethyamine (70:30:0.2:0.4) as mobile phase with a flow rate of 0.4 mL/min of OND. The column temperature was set at 20 degrees C. Baseline separation was achieved for the resolution of the two chiral drugs under the above chromatographic conditions. The proposed method was simple, reproducible and less-time consuming.