A spin cartridge system for DNA extraction from paraffin wax embedded tissues

被引：12
|
作者：
Pinto, AP
Villa, LL
机构：
[1] Ludwig Inst Canc Res, Dept Virol, BR-01509010 Sao Paulo, Brazil
[2] Univ Fed Parana, Dept Pathol, BR-80060000 Curitiba, Parana, Brazil
来源：
JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY | 1998年 / 51卷 / 01期
关键词：
paraffin wax embedded tissues; DNA extraction; polymerase chain reaction; human papillomavirus;
D O I：
10.1136/mp.51.1.48
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
A simple and efficient method of DNA extraction from paraffin wax embedded tissues using a spin cartridge system is described. Such DNAs were shown to be suitable for amplification by the polymerase chain reaction, which targeted two human papillomavirus genes and one globin fragment giving rise to products of 450, 150, and 110 base pairs, respectively. Different human tissues, stored for up to 20 years, were successfully amplified, demonstrating the usefulness of this very simple procedure for retrospective studies.
引用
收藏
页码：48 / 49
页数：2
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