Studies on the propagation of Fritillaria camtschatcensis Ker-Gawl. by in vitro culture. I. Characteristics of sprouting, leafiness, flowering and new bulb formation from mother bulb, and bulblet formation from small globule-scale
bulblet;
Fritillaria camtschatcensis;
in vitro propagation;
NAA;
24-epibrassinolide;
D O I:
暂无
中图分类号:
S6 [园艺];
学科分类号:
0902 ;
摘要:
Wild black lily bulbs were planted in pots and grown for 8 months in a glasshouse. The bulbs produced scapes with 1 similar to 2 terminal flowers and 5 similar to 6 leaves with a verticillate phyllotaxis. Tow new bulbs were formed on;he mother-bulb as it was consumed and eventually rotted. Morphological characteristics matched those of Fritillaria camtschatcensis Ker-Gawl. Small globule-scales of the bulbs were excised and cultured on MS-media without plant hormones in the dark or under light of 40 mu mol.m(-2).sec(-1) al 15, 20, and 25 degrees C for 8 weeks. The optimum condition for bulblet formation was a MS-medium containing a combination of 0.1 mg.liter(-1) NAA and 0.1 mg.liter(-1) 24-epibrassinolide (EB) at 20 degrees C in the dark.