Application of capillary electrophoresis for DNA-protein binding tests

被引：1
|
作者：
Kazmierczak, A
Kazmierczak, JM
机构：
[1] Univ Lodz, Dept Cytophysiol, PL-90231 Lodz, Poland
[2] Univ Lodz, Dept Cytogenet & Plant Mol Biol, PL-90237 Lodz, Poland
来源：
ACTA PHYSIOLOGIAE PLANTARUM | 2003年 / 25卷 / 01期
关键词：
affinity chromatography; capillary electrophoresis; DNA-protein binding; ds-; ssDNA;
D O I：
10.1007/s11738-003-0031-2
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The combination of affinity chromatography and capillary electrophoresis (CE-SDS) has been found to be a useful tool to analyse populations of proteins which specifically bind to ssor dsDNA. Proteins were extracted from tissue, cytosol or nuclei of meristems of Pisum sativum seedlings and separated on cellulose column functionalized with ss-, dsDNA (calf thymus) and ssDNA (P. sativum) at 2M concentration of sodium chloride. Electropherograms of the crude protein extracts show two fractions of proteins specific for dsDNA (calf thymus) and three fractions specific for ssDNA (calf thymus). Four and five fractions of proteins specific for ssDNA (P. sativum) were identified in the material isolated from cytosolic and nuclear extracts, respectively. Both ds- and ssDNA (calf thymus) form complexes with ca. 4.0 % of the total amount of proteins, while ssDNA (P. sativum) binds to ca. 11.0 % of cytosolic and 5.0 % of nuclear proteins.
引用
收藏
页码：13 / 17
页数：5
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