Determination of uracil in 5-fluorouracil substance by high-performance liquid chromatography

被引：4
|
作者：
Kazoka, H [1 ]
机构：
[1] Latvian Inst Organ Synth, LV-1006 Riga, Latvia
来源：
JOURNAL OF CHROMATOGRAPHY A | 2003年 / 994卷 / 1-2期
关键词：
mobile phase composition; uracil; fluorouracil; pyrimidines; butylbenzene;
D O I：
10.1016/S0021-9673(03)0042-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Separation of 5-fluorouracil and uracil in chromatographic systems consisting of a silica column as a stationary phase and ethyl acetate or ethyl acetate partially saturated with water as a mobile phase has been studied. The results indicated that when saturation of ethyl acetate with water was chosen to reach more than 60%, such mobile phases [e.g., 2.4% (v/v) water in ethyl acetate] are useful in determining uracil in 5-fluorouracil substances. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：221 / 225
页数：5
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