A β-glucan-conjugate vaccine and anti-β-glucan antibodies are effective against murine vaginal candidiasis as assessed by a novel in vivo imaging technique

被引：61

作者：

Pietrella, Donatella ^{[1
]}

Rachini, Anna ^{[1
]}

Torosantucci, Antonella ^{[2
]}

Chiani, Paola ^{[2
]}

Brown, Alistair J. P. ^{[3
]}

Bistoni, Francesco ^{[1
]}

Costantino, Paolo ^{[4
]}

Mosci, Paolo ^{[1
]}

d'Enfert, Christophe ^{[5
,6
]}

Rappuoli, Rino ^{[4
]}

Cassone, Antonio ^{[2
]}

Vecchiarelli, Anna ^{[1
]}

机构：

[1] Univ Perugia, Dept Expt Med & Biochem Sci, Microbiol Sect, I-06122 Perugia, Italy

[2] Ist Super Sanita, Dept Infect Parasit & Immune Mediated Dis, I-00186 Rome, Italy

[3] Univ Aberdeen, Sch Med Sci, Aberdeen AB25 2ZD, Scotland

[4] Novartis Vaccines & Diagnost Res Ctr, Siena, Italy

[5] Inst Pasteur, Unite Biol & Pathogenicite Fong, F-75015 Paris, France

[6] INRA, USC2019, F-75015 Paris, France

来源：

VACCINE | 2010年 / 28卷 / 07期

关键词：

Vaginal infection; Candida; MF59; In vivo imaging; INVASIVE FUNGAL-INFECTIONS; MANNOSE-BINDING LECTIN; ALBICANS VAGINITIS; GENE POLYMORPHISM; HOST-DEFENSE; RAT MODEL; PROTECTION; MUCOSAL; MICE; IMMUNITY;

D O I：

10.1016/j.vaccine.2009.12.021

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The protective capacity of a parenterally administered beta-glucan-conjugate vaccine formulated with the human-compatible MF59 adjuvant was assessed in a murine model of vaginal candidiasis. To monitor infection, an in vivo imaging technique exploiting genetically engineered, luminescent Candida albicans was adopted, and compared with measurements of colony forming units. The vaccine conferred significant protection, and this was associated with production of serum and vaginal anti-beta-glucan IgG antibodies. Vaginal IgG molecules were the likely mediators of protection as inferred by the efficacy of passive transfer of immune vaginal fluid and passive protection by an anti-beta-1,3-glucan mAb. Overall, the in vivo imaging technique was more reliable than vaginal CFU counts in assessing the extent and duration of the vaginal infection, and the consequent protection level. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1717 / 1725

页数：9