Development of TaqMan RT-qPCR for the detection of type A human respiratory syncytial virus

被引：5
|
作者：
Abdel-Moneim, Ahmed S. [1 ,2 ]
Shehab, Gaber M. [3 ,4 ]
Alsulaimani, Adnan A. [5 ]
Al-Malky, Mater I. R. [6 ]
机构：
[1] Taif Univ, Coll Med, Div Virol, Dept Microbiol, Al Taif 21944, Saudi Arabia
[2] Beni Suef Univ, Fac Vet Med, Dept Virol, Bani Suwayf 62511, Egypt
[3] Taif Univ, Coll Med, Dept Biochem, Al Taif 21944, Saudi Arabia
[4] Cairo Univ, Fac Agr, Dept Biochem, Giza 12613, Egypt
[5] Taif Univ, Coll Med, Dept Paediat, Al Taif 21944, Saudi Arabia
[6] Hosp Paediat, Al Taif 21944, Saudi Arabia
来源：
MOLECULAR AND CELLULAR PROBES | 2017年 / 33卷
关键词：
RT-qPCR; hRSV-A; Saudi Arabia; Virus detection; Assay development; Human respiratory syncytial virus; DISEASE SEVERITY; INFECTION; PCR; DIVERSITY; INFANTS; GENE; LOAD;
D O I：
10.1016/j.mcp.2017.02.006
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The human respiratory syncytial virus is a common respiratory pathogen in children. Improved diagnosis of the virus is dependent on the development of tools for the rapid detection and estimation of the viral loads. In the current study, RT-qPCR using TaqMan hydrolysis probe based on the F gene detection was developed to identify and quantify hRSV in clinical samples. The assay was validated by comparing the results with a commercially available RT-qPCR kit. The newly developed assay was sensitive in detecting hRSV positive samples (59/126) which were equivalent to those detected by the commercial kit (57/126) with a detection limit of 1 x 10(2) copies/mL. A high correlation was found between the results of the newly developed assay and the commercial one. It was concluded that the newly developed RT-qPCR assay can be used as a sensitive detection tool for hRSV-A. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：16 / 19
页数：4
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