Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations

被引：26
|
作者：
Espada, Caroline R. [1 ]
Ortiz, Paola A. [1 ]
Shaw, Jeffrey J. [1 ]
Barral, Aldina M. P. [2 ]
Costa, Jackson M. L. [2 ]
Uliana, Silvia R. B. [1 ]
Coelho, Adriano C. [1 ,3 ]
机构：
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508900 Sao Paulo, SP, Brazil
[2] Fundacao Oswaldo Cruz FIOCRUZ, Inst Goncola Moniz, BR-40296710 Salvador, BA, Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Biol Anim, BR-13083862 Campinas, SP, Brazil
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2018年 / 91卷 / 04期
基金：
巴西圣保罗研究基金会;
关键词：
Leishmania (Leishmania) amazonensis; Leishmania (Viannia) species; hsp70; PCR-RFLP; molecular diagnosis; MUCOCUTANEOUS LEISHMANIASIS; AMAZONIAN BRAZIL; NEW-WORLD; VIANNIA; PARASITE; CLASSIFICATION; POLYMORPHISM; TAXONOMY; SAMPLES; REGION;
D O I：
10.1016/j.diagmicrobio.2018.03.004
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Hsp70 is a cytoplasmic heat-shock protein, encoded by a multicopy tandemly repeated gene that has recently been gaining popularity as a valuable marker for typing Leish mania species. In this study, we used a previously described hsp70 PCR-RFLP method for identifying Brazilian Leishmania isolates. We identified two distinct L (L) amazonensis hsp70 alleles that resulted in two different RFLP patterns. Also, we found RFLP polymorphisms amongst L (Viannia) naiffi strains. The profiles of both L (V.) shawi and L (V.) lindenbergi were very similar to those of other L (Viannia) species. The observations described herein reflect the polymorphism found within species of Leishmania and indicate that results from this hsp70 PCR-RFLP method should be used with caution when typing isolates from clinical cases of leishmaniasis and Leishmania species from Brazil. (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码：312 / 318
页数：7
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