Development and validation of a micellar liquid chromatographic method with UV detection for determination of azithromycin in tablets and capsules

A rapid and simple micellar liquid chromatographic method that does not require use of specific chromatographic columns has been developed and validated for azithromycin determination. The method uses a Hypersil C-18 column at 60 degreesC, 1-butanol-pH 6.86 phosphate buffer solution-water, 15:25:60 (v/v), containing 0.10 m sodium dodecyl sulfate, as mobile phase, and UV-detection at 215 nm. Dierent characteristics of the method were validated satisfactorily. The specificity, accuracy, linearity, precision (repeatability), and robustness of the method were demonstrated. The method proved suitable for determination of the azithromycin content of capsules and uncoated tablets.