Single-shot Fresnel incoherent correlation holography microscopy with two-step phase-shifting

Fresnel incoherent correlation holography technology can not only combine other techniques for three-dimensional imaging of objects but also has the inherent ability to create a super-resolution reconstruction. It generally realizes reconstructions of objects through phase-shifting algorithm, so it is not appropriate for the real-time imaging of living cells. We propose a single-shot microscopic imaging method, which adjusts the pixel distribution of the SLM based on a two-step phase-shifted mask to make sure beams can travel along their respective optical axis, then the multiplexed phase-shifted hologram can be obtained simultaneously. Experimental reconstructions of some objects are used to verify the effectiveness of our proposal. It is hopeful to realize the real-time imaging of living cells by using this method.