Application of Struvite Alters the Antibiotic Resistome in Soil, Rhizosphere, and Phyllosphere

被引:219
|
作者
Chen, Qing-Lin [1 ,2 ]
An, Xin-Li [1 ,2 ]
Zhu, Yong-Guan [1 ,3 ]
Su, Jian-Qiang [1 ]
Gillings, Michael R. [4 ]
Ye, Zhi-Long [1 ]
Cui, Li [1 ]
机构
[1] Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, 1799 Jimei Rd, Xiamen 361021, Peoples R China
[2] Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
[3] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China
[4] Macquarie Univ, Dept Biol Sci, Sydney, NSW 2109, Australia
基金
中国国家自然科学基金; 对外科技合作项目(国际科技项目);
关键词
SWINE WASTE-WATER; HORIZONTAL GENE-TRANSFER; RESISTANCE GENES; SEWAGE-SLUDGE; PHOSPHORUS RECOVERY; GREENHOUSE EVALUATION; MANURE FERTILIZATION; BACTERIAL COMMUNITY; LAND APPLICATION; ABUNDANCE;
D O I
10.1021/acs.est.7b01420
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Struvite recovered from wastewater is a renewable source of phosphorus and nitrogen and can be used as fertilizer for plant growth. However, antibiotics and resistome can be enriched in the struvite derived from wastewater. Robust understanding of the potential risks after struvite application to soils has remained elusive. Here, we profiled antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in struvite, soil, rhizosphere and phyllosphere of Brassica using high-throughput quantitative PCR. A total of 165 ARGs and 10 MGEs were detected. Application of struvite was found to increase both the abundance and diversity of ARGs in soil, rhizosphere and phyllosphere. In addition, ARGs shared exclusively between Brassica phyllosphere and struvite were identified, indicating that struvite was an important source of ARGs found in phyllosphere. Furthermore, OTUs shared between rhizosphere and phyllosphere were found to significantly correlate with ARGs, suggesting that microbiota in leaf and root could interconnect and ARGs might transfer from struvite to the surface of plants via rhizosphere using bacteria as spreading medium. These findings demonstrated that struvite as an organic fertilizer can facilitate the spread of antibiotic resistance into human food chain and this environment-acquired antibiotic resistance should be put into human health risk assessment system.
引用
收藏
页码:8149 / 8157
页数:9
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