Canceration and thermal coagulation of human liver induced changes in the absorption and scattering properties of liver-tissue at near infrared in vitro

Canceration and thermal coagulation of human liver induced changes in the absorption and scattering properties of liver tissue at 710, 730, 750, 77, 790, 810, 830, 850, 870 and 890 nm of Ti: sapphire laser were studied in vitro. The measurements were performed using a double-integrating-sphere setup, and the absorption and scattering properties were assessed from these measurements using the inverse adding-doubling method. The results of measurement showed that canceration of liver induced significant decrease in the absorption coefficients of liver tissue, and the maximum change in the absorption coefficients is 86.12% at 850 nm, while the minimum change in the absorption coefficients is 82.65% at 750 nm. Thermal coagulation of normal liver induced obvious change in the absorption coefficients from 710 to 890 nm, and the maximum change in the absorption coefficients is 79.55% at 710 nm, while the minimum change in the absorption coefficients 0.72% at 790 nm. Thermal coagulation of carcinoma liver tissue induced significant increase in the absorption coefficients, the maximum change in the absorption coefficients of carcinoma liver tissue is 78.69% at 810 nm, in the minimum change in the absorption coefficients of carcinoma liver tissue 38.16% at 710 nm. Canceration of liver induced significant increase in the scattering coefficients of liver tissue, and the maximum change in the scattering coefficients is 158.37% at 710 nm, while the minimum change in the scattering coefficients is 136.03% at 890 nm Thermal coagulation of normal liver induced significant increase in the scattering coefficients of liver tissue, and the maximum change in the scattering coefficients is 632.92% at 890 nm, while the minimum change for the scattering coefficients is 587.40% at 710 nm. Thermal coagulation of carcinoma liver tissue induced significant increase in the scattering coefficients, and the maximum change in the scattering coefficients of carcinoma liver tissue is 384.25% at 810 nm, while the minimum change in the scattering coefficients of carcinoma liver tissue is 330.86% at 710 nm The change in the absorption and scattering properties also varies with the change of laser wavelength.