Mass Spectrometric Evidence for the Existence of Distinct Modifications of Different Proteins by 2(E),4(E)-Decadienal

被引：13
|
作者：
Zhu, Xiaochun [1 ]
Tang, Xiaoxia [1 ]
Zhang, Jianye [1 ]
Tochtrop, Gregory P. [1 ]
Anderson, Vernon E. [2 ]
Sayre, Lawrence M. [1 ]
机构：
[1] Case Western Reserve Univ, Dept Chem, Cleveland, OH 44106 USA
[2] Case Western Reserve Univ, Dept Biochem, Cleveland, OH 44106 USA
来源：
CHEMICAL RESEARCH IN TOXICOLOGY | 2010年 / 23卷 / 03期
关键词：
LIPID-PEROXIDATION PRODUCTS; COLLISIONALLY ACTIVATED DISSOCIATION; ASSISTED-LASER-DESORPTION/IONIZATION; N-ALKYLPYRIDINIUM CATIONS; COVALENT MODIFICATION; CROSS-LINK; NUCLEOPHILIC DISPLACEMENTS; OXIDATIVE STRESS; LEAVING GROUPS; CYTOCHROME-C;
D O I：
10.1021/tx900379a
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
2(E),4(E)-Decadienal (DDE), a lipid peroxidation product, was found to covalently modify Lys residues of different proteins by different reactions using mass spectrometry (MALDI-TOF-MS and LC-ESI-MS). DDE mainly formed Lys Schiff base adducts with cytochrome c and ribonuclease A at 10 min, but these reversibly formed adducts almost disappeared after 24 h. In contrast, beta-lactoglobulin (beta-LG) was highly modified by DDE after 24 h. In addition to the Lys Schiff base adducts, DDE formed novel Lys pyridinium adducts as well as Cys Michael adducts with beta-LG.
引用
收藏
页码：467 / 473
页数：7
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