Occurrence of Coxiella burnetii in Polish dairy cattle herds based on serological and PCR tests

被引：11
|
作者：
Szymanska-Czerwinska, Monika [1 ,2 ]
Jodelko, Agnieszka [1 ]
Niemczuk, Krzysztof [1 ,2 ]
机构：
[1] Natl Vet Res Inst, Dept Cattle & Sheep Dis, PL-24100 Pulawy, Poland
[2] Natl Vet Res Inst, Lab Serol Diag, PL-24100 Pulawy, Poland
来源：
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2019年 / 67卷
关键词：
Coxiella burnetii; Q fever; Serology; Real-time PCR; Daily cattle; Q-FEVER; RAW-MILK; SEROPREVALENCE; PREVALENCE;
D O I：
10.1016/j.cimid.2019.101377
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The aim of the research was to assess the prevalence of antibodies to Coxiella burnetii in dairy cattle herds in Poland and to compare the results of real-time PCR and ELISA tests performed on bulk tank milk (BTM) samples. In total, 2635 serum samples collected from 969 dairy cattle herds from all provinces were tested using ELISA. Additionally, BTM specimens from 101 herds were analysed by ELISA and real-time PCR targeting IS1111 element. Presence of anti-C. burnetii antibodies was confirmed in 25.39% of serum samples in 237 herds (24.46%) and the herd-level seroprevalence in Voivodeships varied from 2.5% to 61.4%. Moreover, 46 (45.5%) of analysed bulk tank milk samples gave postive result in ELISA and microbial DNA was detected in 40 (39.6%) of tested herds. The comparative analysis of ELISA and real-time PCR results obtained for BTM samples using the chi-square test showed statistically significant relationship between results of both methods.
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页数：6
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