Silencing hsa_circ_0007841 Inhibits Cell Proliferation and Promotes Cell Apoptosis via Regulating miR-507 in Multiple Myeloma Cells

被引：0
|
作者：
Liu, Jie [1 ]
Wang, Yi [2 ]
机构：
[1] Shanxi Med Univ, Dept Hematol, Affiliated Peoples Hosp, Shanxi Prov Peoples Hosp, Taiyuan 030012, Shanxi, Peoples R China
[2] Shanxi Med Univ, Shanxi Prov Peoples Hosp, Affiliated Peoples Hosp, Dept Rheumatol, Taiyuan 030012, Shanxi, Peoples R China
来源：
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING | 2021年 / 11卷 / 04期
关键词：
hsa_circ_0007841; Cell Proliferation; Cell Apoptosis; miR-507; Multiple Myeloma; CARCINOMA PROGRESSION; PROGNOSTIC BIOMARKER; CIRCULAR RNAS; CANCER-CELLS; IDENTIFICATION; RESISTANCE; MIGRATION; INVASION; SERVES;
D O I：
10.1166/jbt.2021.2581
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Recently study has indicated that hsa_circ_0007841 is up-regulated in patients with multiple myeloma and may act as an important biomarker in Multiple myeloma. However, the mechanisms and effects of hsa_circ_0007841 remain unclear and were firstly investigated herein. The gene expression level was detected via PCR assay. The CCK-8 assay was performed to measure the cell viability. The cell proliferation capacity was evaluated via colony formation assay. The protein express level was detected by western blot and cell apoptosis via flow cytometry. The target of hsa_circ_0007841 was predicted via CircInteractome online tool and validated by luciferase reporter assay. Hsa_circ_0007841 was overexpressed and miR-507 was poorly expressed in multiple myeloma cells. Silencing hsa_circ_0007841 has anti-proliferation and pro-apoptosis effects in multiple myeloma cells. MiR-507 was found to be the target of hsa_circ_0007841. Inhibition of miR-507 relieved the effects of silencing hsa_circ_0007841 in myeloma cells. Silencing hsa_circ_0007841 suppressed cellular proliferative ability and enhanced cell apoptosis rate via targeting and up-regulating miRNA-507 in multiple myeloma cells.
引用
收藏
页码：586 / 594
页数：9
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