Identification of the Biosynthetic Gene Cluster for the Pseudomonas aeruginosa Antimetabolite L-2-Amino-4-Methoxy-trans-3-Butenoic Acid

被引：30
|
作者：
Lee, Xiaoyun [1 ]
Fox, Aine
Sufrin, Janice [2 ]
Henry, Hugues [3 ]
Majcherczyk, Paul [1 ]
Haas, Dieter [1 ]
Reimmann, Cornelia [1 ]
机构：
[1] Univ Lausanne, Dept Microbiol Fondamentale, CH-1015 Lausanne, Switzerland
[2] Roswell Pk Canc Inst, Dept Pharmacol & Therapeut, Buffalo, NY 14263 USA
[3] CHU Vaudois, Lab Cent Chim Clin, CH-1011 Lausanne, Switzerland
来源：
JOURNAL OF BACTERIOLOGY | 2010年 / 192卷 / 16期
关键词：
AMINO-ACIDS; ASPARTATE-AMINOTRANSFERASE; RHIZOBITOXINE; INHIBITION; MECHANISMS; VIRULENCE; PATHWAY; ENZYMES;
D O I：
10.1128/JB.00492-10
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
L-2-Amino-4-methoxy-trans-3-butenoic acid (AMB) is a potent antibiotic and toxin produced by Pseudomonas aeruginosa. Using a novel biochemical assay combined with site-directed mutagenesis in strain PAO1, we have identified a five-gene cluster specifying AMB biosynthesis, probably involving a thiotemplate mechanism. Overexpression of this cluster in strain PA7, a natural AMB-negative isolate, led to AMB overproduction.
引用
收藏
页码：4251 / 4255
页数：5
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