Correlation Analysis of Fluorescence Intensity and Fluorescence Anisotropy Fluctuations in Single-Molecule Spectroscopy of Conjugated Polymers

被引：23

作者：

Thomsson, Daniel ^{[1
]}

Lin, Hongzhen ^{[1
]}

Scheblykin, Ivan G. ^{[1
]}

机构：

[1] Lund Univ, Dept Chem Phys, S-22700 Lund, Sweden

来源：

CHEMPHYSCHEM | 2010年 / 11卷 / 04期

基金：

瑞典研究理事会;

关键词：

chromophores; conjugated polymers; correlation analysis; fluorescence; single-molecule studies; INTRAMOLECULAR ENERGY-TRANSFER; MEH-PPV; SPECTRAL DIFFUSION; ELECTRIC-FIELD; DYNAMICS; BLINKING; CHAINS; CONFORMATION; CHROMOPHORES; ABSENCE;

D O I：

10.1002/cphc.200900724

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Single-molecule spectroscopy techniques are used to investigate time fluctuations of the fluorescence properties of two different types of conjugated polymer, a polythiophene derivative (PDOPT) and a phenylene vinylene derivative (MEH-PPV), at 100 and 293 K. Linear correlation coefficients between fluorescence intensity and polarization are used to characterize fluctuations. We are able to distinguish between different blinking and bleaching effects on the polarization. Further-more, the polarization data reveal clear differences in the topology of these two polymers, which is related to the ordered conformation of the MEH-PPV. Plots of correlation coefficients appear to be very different for the two polymers and are also very sensitive to temperature. These observations prove that correlation analysis is a useful tool to understand fluorescence fluctuations in multi-chromophoric systems.

引用

页码：897 / 904

页数：8

共 50 条

[1] Single-molecule spectroscopy of conjugated polymers
Barbara, PF
Gesquiere, AJ
Park, SJ
Lee, YJ
[J]. ACCOUNTS OF CHEMICAL RESEARCH, 2005, 38 (07) : 602 - 610
[2] Redistribution of emitting state population in conjugated polymers probed by single-molecule fluorescence polarization spectroscopy
Forster, M.
Thomsson, D.
Hania, P. R.
Scheblykin, I. G.
[J]. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2007, 9 (06) : 761 - 766
[3] Single-molecule analysis of restriction DNA fragments using fluorescence correlation spectroscopy
Kinjo, M
Nishimura, G
Koyama, T
Mets, U
Rigler, R
[J]. ANALYTICAL BIOCHEMISTRY, 1998, 260 (02) : 166 - 172
[4] 'True' single-molecule molecule observations by fluorescence correlation spectroscopy and two-color fluorescence cross-correlation spectroscopy
Foeldes-Papp, Zeno
[J]. EXPERIMENTAL AND MOLECULAR PATHOLOGY, 2007, 82 (02) : 147 - 155
[5] Commentary: Single-molecule fluorescence spectroscopy
Patra, Digambara
[J]. JOURNAL OF NANOPHOTONICS, 2012, 6
[6] Measuring complexation by single-molecule fluorescence anisotropy
Burrows, Sean M.
Pappas, Dimitri
[J]. ANALYST, 2008, 133 (07) : 870 - 873
[7] Protein dynamics from single-molecule fluorescence intensity correlation functions
Gopich, Irina V.
Nettels, Daniel
Schuler, Benjamin
Szabo, Attila
[J]. JOURNAL OF CHEMICAL PHYSICS, 2009, 131 (09):
[8] Single-molecule spectroscopy of conjugated polymers.
Barbara, PF
Hu, DH
Yu, J
Song, NW
[J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2001, 221 : U261 - U261
[9] Fluorescence correlation spectroscopy with single-molecule sensitivity on cell and model membranes
Schwille, P
Korlach, J
Webb, WW
[J]. CYTOMETRY, 1999, 36 (03): : 176 - 182
[10] Correlation of vibrational intensity with fluorescence lifetimes in π conjugated polymers
O'Neill, Luke
Lynch, Patrick
McNamara, Mary
Byrne, Hugh J.
[J]. POLYMER, 2008, 49 (19) : 4109 - 4114

← 1 2 3 4 5 →